ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (10): 1899-1904.doi: 10.11843/j.issn.0366-6964.2015.10.025

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Identification of the Interaction of Avian Influenza Virus PA-N182 and COPD Protein of Chicken

WANG Qiao,LI Qing-he,LIU Ran-ran,ZHENG Mai-qing,WEN Jie,ZHAO Gui-ping*   

  1. (Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
  • Received:2015-01-21 Online:2015-10-23 Published:2015-10-23

Abstract:

Host fators are widely involved in transcription,duplication and translation of influenza virus genome,and host factors are reruired for the infection of influenza virus.PA-N182 is a recently identified trunctated form of influenza PA gene,and little is known about its host interaction factors.COPD(coatomer protein complex,subunit delta),with the function of translocation of newly translated peptides from endoplasmic reticulum to Golgi apparatus,has an essential role in duplication of influenza virus and its knock down results in decrease of virus titer.We co-transfected PA-N182 and COPD expression vectors into chicken DF1 cells and found that PA-N182 interacted with COPD by the method of immunoprecipitation and Western blot.Our results reveal that COPD might affect the infection of influenza virus through ineracting with PA-N182 and translating it from endoplasmic reticulum to Golgi apparatus.

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